Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVIDALRKRRAVKRFDPAFQLSENDKKQLLQEVLANAPSAFNLQHWRPVIVEDAELRQKIRAIAW--------------DQPQVTESSLLIVLCAKVNTWEVDAKRVWDGASPEVQDIMVGAIDQYYRDRPQTQRDEVMRSAGIFAQTLMLLAQEHGLDSCPMDGFDFDAMAKLINLPEDHVVCLMIAVGKSASEPYLRVG--KLPYDDVIIQNTF 4G8S Chain:A ((24-206)) -DTLEAIRTRRSVRAFSD--RPVEPEKLQMVLEAARQAPSWANMQCSRFVVVQDAEVKAKISELSFVEAFFAPLGYRTNPAQKALAEAPVVIVAC---------------GVPGESGDLRG---QQYY-----------MTDVGIATENLMLAAHAVGLGSVFVGVFGEEQLGDLLDIPPEIRIVGLFPLGYPR--------PARKPLDEIVFQG--

General information: TITO was launched using: RESULT: Template: 4G8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 770 -47736 -61.99 -300.22 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -61.99 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_4G8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G8S-query.scw PDB file : Tito_Scwrl_4G8S.pdb: