Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAMLPEWTTACPDWEERIVAKKSLMPCEPLFPQVADVAERIFKELILVDVMGSPKMGDVTLDWVIEFVRAIFGSYNPNTKRRLIREFFLLISKKNTKSTIAAGVMLVALLLNDRLSAELIILAPTKEVADNSFNPIRDFIRADEELSAMI-N-VSEH---------TKTVTH----LGTGATLKVIAAE-SNAAAGKKASIILIDEVWLFGKRANAESMF-REAKGGLASRPEGCVIYLSTMSDEVPCGVFKQLLDYARDVRDGIKEDKGFLPLIYEFPKRLVEAGEHLKPEHFYITNPNLGASVDLEYLISEFKKVKDAGEESLRDFLAKHLNIEIGMNLRANRWAGAEYWNKQKHVFGLDQLIEQSDVITIGIDGGGLDDLLGYAVLGRLKKDPRVWWLWNHAWANKIALERRKENIPKYDDFKLEGSLTVVDRVGDDIDQLAAIAKKVYDSGKLNKIGLDPLGLGGLLDGLLEAGIPEESMFAVPQGYKLMSYILTTERKLAEGNLYHAGQQLMTWAAGNARVVMVGNGMRITKQESGVGKIDPLIATFNAVALMSMNPEPTNKEYNVFFV 4ZNK Chain:A ((21-198)) ----------------------------------------------------------YKPHHVQLAIHRST------------AKRRVACLGRQSGKSEAASVEAVFELFA--RPGSQGWIIAPTYDQAEIIFGRVVEKVERLAEVFPATEVQLQRRRLRLLVHHYDRPVNAPGAKRVATSEFRGKSADRPDNLRGATLDFVILDEAAM----IP-FSVWSEAIEPTLSV-RDGWALIISTPKGL---NWFYEFFLMG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZNK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 768 -63426 -82.59 -393.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -82.59 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_4ZNK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZNK-query.scw PDB file : Tito_Scwrl_4ZNK.pdb: