Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLIQPELFFSALSPAEQRIQQALEDIRQGKPVLVMDDFDRENEADLIVAAETLTVETMVRMIHDGSGIVCLCLTEELADHLELPPMVSQNSSQFHTAFTVTIEAAQGVTTGVSAKDRVTTIKTAIKDGAVASDLNRPGHVFPLRARNGGVLTRRGHTEGTIDLARLAGLKPAGVLCELTN--PDGTMASGIQVLAYAQTHHLTVITIEELVQYRQQHGI 3MIO Chain:A ((7-205)) --------------------VERAVADIAAGKAVIVIDDEDRENEGDLIFAAEKATPEMVAFMVRYTSGYLCVPLDGAICDRLGLLPMY-----------TVTVDARNGIGTGISASDRATTMRLLADPTSVADDFTRPGHVVPLRAKDGGVLRRPGHTEAAVDLARMAGLQPAGAICEIVSQKDEGSMAHTDELRVFADEHGLALITIADLIEWRRKH--

General information: TITO was launched using: RESULT: Template: 3MIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1048 -93536 -89.25 -502.88 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -89.25 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.615

(partial model without unconserved sides chains): PDB file : Tito_3MIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MIO-query.scw PDB file : Tito_Scwrl_3MIO.pdb: