Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPWLYWTLKPKHRIWAEEWQKEYQAYLMDMETVEIGENCFISPLAHIFAEPGRKIKIGDNCFIAADCSLHGPLEIGNEVAINHHCILDGGRAGIKLHDQVRIAAYCHLYAF------DHGMQLDRPLYQQPVRSQGIEIEKDVWLGAHVGIKDGIKIGKHAVVGMNSMVTKDVEPYHIVGGNPAKFIRLRE 3VBI Chain:A ((24-189)) -----------------------QEELKKIGFLSVGKNVLISKKASIY-NPGV-ISIGNNVRIDDFCILSGKVTIGSYSHIAAYTALYGGEVGIEMYDFANISSRTIVYAAIDDFSGNALMGPTIPNQYKNVKTGKVILKKHVIIGAHSIIFPNVVIGEGVAVGAMSMVKESLDDWYIYVGVPVRKIKARK

General information: TITO was launched using: RESULT: Template: 3VBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 876 -131477 -150.09 -821.73 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -150.09 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3VBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VBI-query.scw PDB file : Tito_Scwrl_3VBI.pdb: