Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFNQLQIALGTLVRKEVRRFLRIWPQTLLPPAITMSLYFVIFGNLVGSRIGEMGGVSYMQFIVPGL--IMMAVITNSYANVSSSFFSVK----FQKNIEELIMSPVPLHIILWG-YVIGGICRGVLVGAIVTAMS--MFFTD--LFIHNWFVTIYTVLI-------------------------TSVLFSLGGFINAVYAKSFDDISIIPTFVLT--------PLTYLGG----VFYAISALGPFWQKLSLINPIVYMVNAFRFGILGHSDVNVSFSLIIVTLCCAVL---YGIAYYLLARGSGMRE------------ 4EHX Chain:A ((1-315)) -MLRSSLLPFSYLYEKIINFRNTLYDKGFLKIK-KLPVPVISVGNLS---VGGSGKTSFVMYLADLLKDKRVCILSRGYKRKSKGTLIVSEYGNLKVSWEEAGDEPYLMAKLLPHVSVVASEDRYKGGLLALEKLSPEVFILDDGFQHRKLHRDLNILLLKKKDLKDRLLPAGNLREPLKEIRRADALVLTYQEVEPFEFFTGKPTFKMFREFCCLLNSDFEEVPFDILKEREVIAFSGLGDNGQFRKVLKNLGIKVKEFMSFPDHYDYSDFTPEEGEIYLTTPKDLIKLQGYENVFALNFKVKLEREEKLKKLIYRIFY

General information: TITO was launched using: RESULT: Template: 4EHX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1182 -121958 -103.18 -483.96 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -103.18 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_4EHX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EHX-query.scw PDB file : Tito_Scwrl_4EHX.pdb: