Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIAVEKVSAEEARLMSTGFLFPGQGSQKLGMLAD------IPEMYMERLENVTGYRLV-----DKEENYQDTVFIQLALLTKAV-FYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVYHRAVLMKEAYPSGYA-MGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSIEGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKNTDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKFGVDKGIRTINLDQHGIDDATFLYQKWR 3H0P Chain:A ((9-293)) --------------------FVFPGQGSQSVGMLAEMAANYPIVEETFAEASAALGYDLWALTQQGPAEELNKTWQTQPALLTASVALWRVWQQQGGKMPALMAGHSLGEYSALVCAGVINFADAVRLVEMRGKFMQEAVPEGTGGMSAIIGLDDASIAKACEESAE-GQVVSPVNFNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAKITFSAPTVPVVNNVDVKCETDAAAIRDALVRQLYNPVQWTKSVEFIAAQGVEHLYEVGPGKVLTGL--TKRIVD------------------------

General information: TITO was launched using: RESULT: Template: 3H0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1547 794 0.51 2.92 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 0.51 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_3H0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H0P-query.scw PDB file : Tito_Scwrl_3H0P.pdb: