Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASFDQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLLEHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYELFQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKSTYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEIFYQYDLTRSKKLP-TAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVGKIEVYER--DRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVLLMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITFCQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN 1H99 Chain:A ((72-275)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EVSEEIISYAKLQLGKKLNDSIYVSLTDHINFAIQRNQKGLDIKNALL-WETKRLYKDEFAIGKEALVMVKNKTGVSLPEDEAGFIALHIVNAELNEEMP---N------IINITKVMEEILS-IVKYHFKI-EFN--EESLHYYRFV-TDLKFFAQRLFNGTHM-EDDFLLDTVKEKY-HRAYECTKKIQTYIEREY-EHKLTSDELLYLTIDIERVVK---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1H99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 754 -51422 -68.20 -258.40 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -68.20 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_1H99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H99-query.scw PDB file : Tito_Scwrl_1H99.pdb: