Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRES--------SFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLYW--------------VVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK 5I74 Chain:A ((165-306)) -----------------------------------------------------------------------------------------------------------------------------QIHRSKGLQDLGGISWQLALCIMLIFTVIYFS--IWKGVKTSGKVVWVTATFPYIAL---SVLLVRGATLPGAWRGVLFYLK-PNWQ-KLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVI---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5I74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 93 -7986 -85.87 -66.55 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -85.87 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : -0.127

(partial model without unconserved sides chains): PDB file : Tito_5I74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I74-query.scw PDB file : Tito_Scwrl_5I74.pdb: