Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFSNGAISESTSEMEKASRMSEINFGVSLVFWGKQEIS 1Z65 Chain:A ((1-30)) MKNRLGTWWVAILCMLLASHLSTVK---------ARGIK

General information: TITO was launched using: RESULT: Template: 1Z65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 12 -435 -36.25 -14.50 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -36.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_1Z65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z65-query.scw PDB file : Tito_Scwrl_1Z65.pdb: