Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANEDIITLGANAASKIV---TEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEVKEACYGGTAALHMAKEYV--KNHPERKVLVIASDIARYGLASGGEV--------TQGVGAVAMMITQNPRILSIE-DDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELSGRGL---EDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIR----YSRRIGNLYTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI 5KP7 Chain:A ((27-395)) -QVGIEALSVYGGAAQLELRKLAQARQLDISRFD-NLMMKEKAVSLPYEDPVSYAVNAAKPIIDRLSDADKQRIEMVITCSESGIDFGKSMSTYIQEYLGLSRNCRMFELKQACYSGTAGLQMAINLILSQTFPGAKALVIATDISRFLV------YDWSFAEPSSGAGAVALLVSDTPHIFQIDVGCNGYYGYEVMDTCRPN-PDSEAGDADLSLLSYLDCCENAY-RHYQNRVEGVDYRESFDYLSFHTPFGGMVKGAHRNMMRRLKRAKPAEIEADFQRRVMPGLVYCQQVGNIMGATLFLSLASTIDNGDFSTP-RRIGMFSYGSGCCSEFYSGVVTPEGAAIAAQQGISAQLADRYSLSMEEYEQLL---------------------------------

General information: TITO was launched using: RESULT: Template: 5KP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2108 -9524 -4.52 -28.01 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -4.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_5KP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KP7-query.scw PDB file : Tito_Scwrl_5KP7.pdb: