Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGGGVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIVKNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI 5ADY Chain:L ((2-143)) -RLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGV-VDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIE--

General information: TITO was launched using: RESULT: Template: 5ADY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 412 -32311 -78.42 -227.54 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain L : 0.80 3D Compatibility (PKB) : -78.42 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_5ADY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ADY-query.scw PDB file : Tito_Scwrl_5ADY.pdb: