Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILVNIEGRLEK-------DDGTEEKKGIQKEVTIDRKAN------LKSHRLYWIGRRAQDVFCSALALIILSPVMLITALAIVIDDLGGSPIFAQNKVSRNGRLFKFYKFCSMCVDAEAKLDALLSQNEMDGPVFKIKNDPRITRVGKFI 3ZFJ Chain:A ((12-130)) ---SYIDGDQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE-ELLMKDPNYQLKDSDIVNEIKGGYVIKV------DGKYYVYLK------------DAAHADN--------IRTKEEIKRQKQ--

General information: TITO was launched using: RESULT: Template: 3ZFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 31021 66.71 292.65 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 66.71 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_3ZFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZFJ-query.scw PDB file : Tito_Scwrl_3ZFJ.pdb: