Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFFQGHFPEEPVMPGVLIVEAMAQAGA-VALLSLEQF-----KGKTAYFGGLDKAKFRKKVTPGDTLYLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG 4ZJB Chain:A ((26-168)) --IEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAELKAMIA

General information: TITO was launched using: RESULT: Template: 4ZJB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 626 -43320 -69.20 -316.20 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -69.20 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_4ZJB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZJB-query.scw PDB file : Tito_Scwrl_4ZJB.pdb: