Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINV----GILTLLSVLLAYRHKVILNGPLFASILTVTGFSFF------GKNLYNSISIILGV-----YLYAVFVNK--PFSQYIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAV--GFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR 4QUV Chain:A ((23-427)) ----------FSWLVLMIALPPLVYYLWICVTYYQGELVFTSDAAAWRRFWSHVAPPTWHAAGLYAAWFLGQAALQVWAPGPTVQGMKLPDGSRLDYRMNGIFSFLFTLAVVFGLVTMGWLDATVLYDQLGPLLTVVNIFTFVFAGFLYFWGPFYDYFM----GTALNPRIG--------SLDLKLFCEARPGMIFWLLMNLSMAAKQYELHGTVTVPMLLVVGFQSFYLIDYFIHEEAVLTTWDIKHEKFGWMLCWGDLVWLPFTYTLQAQYLVHHTHDLP---------------------VWGIIAIVALNLAGYAIFRGANIQKHHFRRDPNRIVWGKPAKYIKTKQGSLLLTS-------GWWGIARHMNYFGDLMIALSWCLPAAF-GSPIPYFHIVYFTILLLHREKRDDAMCLAKYG--------------EDWLQYRKK---VPWRIVPKIY-

General information: TITO was launched using: RESULT: Template: 4QUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1566 -216429 -138.20 -578.69 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -138.20 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_4QUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QUV-query.scw PDB file : Tito_Scwrl_4QUV.pdb: