Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNGA-EIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRL--RIYYAKTSV--IGNTIEVKAEPIFNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQ-FS---FSSLVD-TLANVKI-EKANFLEFFGGKEGSILDRFHGEFLKDN-NTLRHEKSLGTD-H-KIKAIYTKN-----LTGLDLEIDAQSVLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVL--TNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF 3D37 Chain:B ((19-339)) -----------------------------HRHWERYDIDSDFLIPADSFDFVI--------PDLSGESCEVVID---GQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCSAPQLNVK-----GMTVLDAAKKLAAP---WPQIKAVVLKAENNPALGKIDIEPGETVWQAL----THIANSVGLHPWLEPDGTLVVGGA-DYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTFL-------------LKWVYKDPTMTLHRPK---TVVV----DNLAALQKQAKKQLAD---WRLEGFTLTITVGGHKTR-------DGVLWQPGLRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELRLKEDG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3D37.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1223 15364 12.56 56.07 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : 12.56 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_3D37.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D37-query.scw PDB file : Tito_Scwrl_3D37.pdb: