Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAALHQPSQVQLNQDGNLKHFLTIEGLSKENLTKILDTAQSFLDDNNNLINRPLLEGRTVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPKVAIINAGDGRHAHPTQAMLDMLTIRREMKKPFEDLSVAIIGDIKHSRVARSDVAALQTLGCKDIRVIAPNTL-LPVGFSE----YGDHVRLFNNMDEGITGCDVIIALRIQNERIDSPALSSQSEFYRMYGLNKERLSLAKPDCIVMHPGPMNRGVEIDSSIADGEQSVILKQVTNGIAVRMAVLALSMQGQLQEQGLIDAIAL 5G1N Chain:E ((8-313)) ---------------HSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCY-ADVVVLRHPQPG--AVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREE-LGTVNGMTITMVGDLKHGRTVHSLACLLTQYRV-SLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGST--QEYEACFGQFILTPHIMTRAKKKMVVMHPMPRV--NEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGR-------------

General information: TITO was launched using: RESULT: Template: 5G1N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1633 -112499 -68.89 -373.75 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain E : 0.74 3D Compatibility (PKB) : -68.89 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_5G1N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1N-query.scw PDB file : Tito_Scwrl_5G1N.pdb: