Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYHHLTAINQYFLHSRMFNDWGIEQLGSAEYKESIRQMKHADKIIERILFLEGLPNLQHLGKLYIGQHTEEVLQCDIRKVKENIEAIQKAVALAETEQDYVTRDLVQEILEKEEEYWDWLDTQIDLIGSVGIENYIQSQM 5D8R Chain:D ((14-154)) ILGNELIAINQYFLHSRMWNDWGLKRLGAHEYHESIDEMKHADKLIERILFLEGLPNLQDLGKLLIGANTQEMLQCDLNLELKATKDLREAIVHCEQVHDYVSRDLLKDILESEEEHIDYLETQLGLIQKVGLENYLQSHM

General information: TITO was launched using: RESULT: Template: 5D8R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 504 -43666 -86.64 -309.69 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain D : 0.91 3D Compatibility (PKB) : -86.64 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_5D8R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D8R-query.scw PDB file : Tito_Scwrl_5D8R.pdb: