Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRG-VKLKGCPNEPNFGSLSALVYQH 2LQN Chain:A ((17-88)) GPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSEN-----------------SRVSHYIINSLPNRRFKI-G---DQEFDHLPALLEFY

General information: TITO was launched using: RESULT: Template: 2LQN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 258 -21048 -81.58 -296.44 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -81.58 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_2LQN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LQN-query.scw PDB file : Tito_Scwrl_2LQN.pdb: