Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSG-YAFPGGHVENDEAFAESVIREIYEETGLTIQNPQLV--GIKNWPLDTGGRYIVICYKATEFSGTLQSSEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF 3R03 Chain:A ((6-136)) -----PILLVTAAALID-PDGRVLLAQRPPG-KSLAGLWEFPGGKLEPGETPEAALVRELAEELGVDTRASCLAPLAFASHSY-DTFHLLMPLYACRSWRGRATAREGQTLAWVRAERLREYPMPPADLPLIPILQDWL-------------------

General information: TITO was launched using: RESULT: Template: 3R03.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 626 -29747 -47.52 -232.40 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -47.52 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_3R03.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R03-query.scw PDB file : Tito_Scwrl_3R03.pdb: