Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAALHQPSQVQLNQDGNLKHFLTIEGLSKENLTKILDTAQSFLDDNNNLINRPLLEGRTVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPKVAIINAGDGRHAHPTQAMLDMLTIRREMKKPFEDLSVAIIGDIKHSRVARSDVAALQTLGCKDIRVIAPNTL-LPVGFSE----YGDHVRLFNNMDEGITGCDVIIALRIQNERIDSPALSSQSEFYRMYGLNKERLSLAKPDCIVMHPGPMNRGVEIDSSIADGEQSVILKQVTNGIAVRMAVLALSMQGQLQEQGLIDAIAL 5G1O Chain:F ((5-313)) ------------PLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLS---------KGESLADSVQTMSCY-ADVVVLRHPQPG--AVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREE-LGTVNGMTITMVGDLKHGRTVHSLACLLTQYRV-SLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGST--QEYEACFGQFILTPHIMTRAKKKMVVMHPMPRV--NEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGR-------------

General information: TITO was launched using: RESULT: Template: 5G1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1554 -111231 -71.58 -377.05 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain F : 0.74 3D Compatibility (PKB) : -71.58 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_5G1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1O-query.scw PDB file : Tito_Scwrl_5G1O.pdb: