Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGIGIAAPQVGQNKRIAVIEVDEG----EKFELINPEIIEAKGES-LDVEGCLSIPHVYGTVKRADEVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIP-EEELEEYMEEHLDD 4DR8 Chain:C ((19-177)) --LDLHYLGDRVLRQPAKRVSRIDDELRQTIRQMLQTMYSADGIGLAAPQVGINKQLIVIDLELEDEQAPPLVLINPKIERTAGDLEQCQEGCLSIPGVYLDVERPEIVEVSYKDENGRPQRLVADGLLARCIQHEMDHLNGVLFVDRVENRLELNEALDK--------

General information: TITO was launched using: RESULT: Template: 4DR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 720 -20590 -28.60 -134.57 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -28.60 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_4DR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DR8-query.scw PDB file : Tito_Scwrl_4DR8.pdb: