Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPLVEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGID--ASALVDHKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGSRATFDTVIMEGQ-SAM--------QSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYDLSPENISSNNWPLWSYEHMYTLGQ-PNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMKVEKDAAGTVTVLEGRQ 1TWY Chain:G ((28-276)) ------------------------------SEITISGSTSVARIMDVLAEKYNQQHPETYVAVQGVGSTAGISLLKKGVADIA----MTSRYLTESEAQNTLHTFTLAFDGLAIVVNQANPVTNLTREQLYGIYKGQITNWKQVGGNDQKIAVVTREASSGTRYSFESLMGLTKTVKDREVSDVAPTALVVNSNSMMKTLVNHNTQAVGFISIGSVDKSVKAIQFEKADPTSDNIAKHTYQLSRPFLILHYSDNADEQTKEFIAFLKSESAK-KLIVEYGYIMP--------------------

General information: TITO was launched using: RESULT: Template: 1TWY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1282 27290 21.29 115.15 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain G : 0.73 3D Compatibility (PKB) : 21.29 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_1TWY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TWY-query.scw PDB file : Tito_Scwrl_1TWY.pdb: