Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGR--------STGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAF-SVFPVGSKVLAVRDLYGGSFRWFNQVEQE--GRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHA-KGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFY--NLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTG----------------RGGMISFKVADE-TRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG 1E5E Chain:A ((4-394)) --ERMTPATACIHANPQ-KDQFGAAIPPIYQTSTFVFDNCQQGGNRFAGQESGYIYTRLGNPTVSNLEGKIAFLEKTEACVATSSGMGAIAATVLTILKAGDHLISDECLYGCTHALFEHALTKFGIQVDFINTAIPGEVKKHMKPNTKIVYFETPANPTLKIIDMERVCKDAHSQEGVLVIADNTFCSPMITNPVDFGVDVVVHSATKYINGHTDVVAGLICGK-ADLLQQIRMVGIKDITGSVISPHDAWLITRGLSTLNIRMKAESENAMKVAEYLKSHPAVEKVYYPGFEDHEGHDIAKKQMRMYGSMITFILKSGFEGAKKLLDNLKLITLAVSLGGCESLIQHPASMTHAVVPKEEREAAGITDGMIRLSVGIEDADELIADFKQGLDA

General information: TITO was launched using: RESULT: Template: 1E5E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2012 -19907 -9.89 -55.30 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -9.89 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_1E5E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5E-query.scw PDB file : Tito_Scwrl_1E5E.pdb: