Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLKAIKESLP--EAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHRDEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAVRAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIASAVAFAIETPDRMSVSNMIIRPTTQEV 3TFO Chain:B ((3-239)) ----MDKVILITGASGGIGEGIARELGVAGAKILLGARRQARIEAIATEIRDAGGTALAQVLDVTDRHSVAAFAQAAVDTWGRIDVLVNNAGVMPLSPLAAVKVDEWERMIDVNIKGVLWGIGAVLPIMEAQRSGQIINIGSIGALSVVPTAAVYCATKFAVRAISDGLRQES--TNIRVTCVNPGVV-----------------------IALQPADIARAVRQVIEAPQSVDTTEITIRPTA---

General information: TITO was launched using: RESULT: Template: 3TFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1179 -63806 -54.12 -299.56 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -54.12 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_3TFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TFO-query.scw PDB file : Tito_Scwrl_3TFO.pdb: