Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAWTPHVTVATVVEKDGRYLFVEEHSEGFVHTVFNQPAGHVECGETLTEAAIRETLEETGHHIDIDALLGIYTYTPPMFPDRTYYRFCFLAHVTHVESDPKLDTGIVSAVWMTLDELKESARARSPLVIKAIEDAMKGQHYPLALIYEHPFSPSLTSHLDA 5X1X Chain:A ((3-126)) ----KVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNETEKDALIREIREEMKCDLIVGDKVITTEHEYDF---GIVRLTTYKCTLNKEL---PTLTEHKSIEWLSINELDK-LNW-APADIPAVNKI--------------------------

General information: TITO was launched using: RESULT: Template: 5X1X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 612 -65702 -107.36 -529.85 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -107.36 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_5X1X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X1X-query.scw PDB file : Tito_Scwrl_5X1X.pdb: