Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAI---YIHDVNF--------AGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTTFRLEPGERLAQYVLVPVVQAEFEQVEEFEETLRGAGGFGHTGKQ 3T64 Chain:B ((1-155)) MHLKIVCLSDEVREMYKNH------DSGLDLFIV--KDEVLKPKSTTFVKLGIKAIALQYK-SNYYYKNIVNTSFLLFPRSSIS-KTPLRLANSIGLIDAGYRGEIIAALDNTSDQEYHIKKNDKLVQLVSFTGEPLSFELVEEL----------------

General information: TITO was launched using: RESULT: Template: 3T64.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 543 -12802 -23.58 -103.24 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -23.58 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_3T64.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T64-query.scw PDB file : Tito_Scwrl_3T64.pdb: