Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQILGGNNMAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS--KKGY-YDGVIFHRVIPDFMIQGGDPT-GTGMGGESIYGESFEDEFSRELFNLRGALSMANSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVLEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE 1W8L Chain:A ((18-142)) ------------------------------GRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAK---------------------------TEWLDGKHVVFGKVKEGMNIVEAM------------------------

General information: TITO was launched using: RESULT: Template: 1W8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 577 15475 26.82 127.89 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 26.82 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_1W8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W8L-query.scw PDB file : Tito_Scwrl_1W8L.pdb: