Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPS-IEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTP-QTFRCKDFMDLYGSLSDEEK-EILTDACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD 3F1C Chain:B ((1-232)) LIYAQILAG-----MGNVSMPKQFLPLNGKPIIVHTVEKFILN-TRFDKILISSPKEWMNHAEDNIKKYIS-DD-RIVVIEGGEDRNETIMNGIRFVEKTYGLTDDDIIVTHDAVRPFLTHRIIEENIDAALETGAVDTVIEALDTIVESSNHEVITDIPVRDHMY-QGQTPQSFNMKKVFNHYQNLTPE-KKQILTDACKICLLAGDDVKLVKGEIFNIKITTPYDLKVANAIIQE-

General information: TITO was launched using: RESULT: Template: 3F1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1108 -30779 -27.78 -137.40 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -27.78 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3F1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F1C-query.scw PDB file : Tito_Scwrl_3F1C.pdb: