Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENF--MTHAKDGY-YNGLTFHRVIKDFMIQGGD-PKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK 5LUD Chain:A ((18-142)) ------------------------------------------------------------------------------GRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANA-GPNTNGSQFFICTAKTEW------------------------LDGKHVVFGKVKEGMNIVEAM-----------------------------

General information: TITO was launched using: RESULT: Template: 5LUD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 587 28453 48.47 235.15 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 48.47 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_5LUD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LUD-query.scw PDB file : Tito_Scwrl_5LUD.pdb: