Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIERTLSIVKPDAVSKNHIGEIFARFEKAGLKIVATKMKHLSQADAEGFYAEHKERGFFGDLVAFMTSGPVVVSVLEGENAVLAHREILGATNPKEAAPGTIRADFAVSIDENAAHGSDSVASAEREIAYFFADNEICPRTR 2NCK Chain:R ((3-140)) --IERTLSIIKPDGLEKGVIGKIISRFEEKGLKPVAIRLQHLSQAQAEGFYAVHKARPFFKDLVQFMISGPVVLMVLEGENAVLANRDIMGATNPAQAAEGTIRKDFATSIDKNTVHGSDSLENAKIEIAYFFRETEIHS---

General information: TITO was launched using: RESULT: Template: 2NCK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 646 -53822 -83.32 -390.01 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain R : 0.90 3D Compatibility (PKB) : -83.32 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2NCK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NCK-query.scw PDB file : Tito_Scwrl_2NCK.pdb: