Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPLVEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGI-D--ASALVDHKVAVAGLALIVNKEV-DVD--NLTTEQLRQIFIGEVTNWKEVG---------GKDLPISVINRAAGSGSRATFDTVIMEGQS--A-----------MQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDD-S---VKSMKLNG--YDLSPENIS---------------------SNNWPLWSYEHMYTLGQ-----PNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMKVEKDAAGTVTVLEGRQ 1IXH Chain:A ((1-309)) -----------------------------EASLTGAGATFPAPVYAKWADTYQK-ETGNKVNYQGIGSSGGVKQIIANTVDFG----ASDAPLSDEKLAQEGLFQFPTVIGGVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRRADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGYVEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWPITSTTFILIHKDQKKPEQGTEVLKFFDWAYKT-GA-KQANDLDYASLPDSVVEQVRAAWK-------

General information: TITO was launched using: RESULT: Template: 1IXH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1354 49282 36.40 197.92 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 36.40 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_1IXH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IXH-query.scw PDB file : Tito_Scwrl_1IXH.pdb: