Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASLSSSPVRHPSVDNAAIPAASHHIPPPLNGYAFLEHRRSALSHDTFIARDVRQQQHPEQQDNDQAKVIIRVYALEYLRRDEECRFALERECLAARLVVHPHLLPLGSPFASKTDLFVVEKYCAGGDLYELMVSLAKEGPIASETGAAKGEAPPRSSTGLPISTVKRFMRELLSAVQYLHRTCGLVHRNIKLETLFIDEEQHLRLGSFGLCAVLPPPSVATGDREGALDAPEDKASSSHAPLRLCCGSKHYAAPELVQGHPYQGEGVDAWACGVVLFALLTGCFPFDSDDGDEALFRLLCGDVESHLAQHPAMAAIEDPQACDLVRNLLRPNPNARYTVSEALEHPYLWEV 4REW Chain:A ((53-389)) -----------------------------------------------------------------------------------------IRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDY---ICKNGRLDEKES-------------------RRLFQQILSGVDYCHRHM-VVHRDLKPENVLLDAHMNAKIADFGLSNMM----------------------SDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFD-DDHVPTLFKKICDGI-FYTPQYL------NPSVISLLKHMLQVDPMKRASIKDIREHEWF---

General information: TITO was launched using: RESULT: Template: 4REW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1003 -154053 -153.59 -740.64 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -153.59 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_4REW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4REW-query.scw PDB file : Tito_Scwrl_4REW.pdb: