Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence TPMLDSLIEHRSPIFCLVQMCEMLDGGKKYCTR------WYKVYPGGSHTFQEGGRDT-ITVTFDNKCKKSLSGSTKKRSIGFPTQLVYRSDDPILAGISKIFEPGRAPNYH 5IQ7 Chain:H ((90-141)) ----------------------MEDSGLYFCARTGKYYDFWSGYPPGEEYFQDWGRGTLVTVS---------SASTKGPSV-FP----------------------------

General information: TITO was launched using: RESULT: Template: 5IQ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 78 3176 40.72 70.58 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain H : 0.63 3D Compatibility (PKB) : 40.72 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_5IQ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IQ7-query.scw PDB file : Tito_Scwrl_5IQ7.pdb: