Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLLGSGRVAFHLAKALLAQGHHIAQVYARDFEKTKKFAEQIQAKACQSLQEFQPTDLIILAVSDSAITELATQVHELF-PKMLMVHTSGSTDIQVISQVH---EKAGVFYPLQTFSLERDVDWKATPLFVEATNKDDLVILSDLANSLSKRVYQYTSKQRLTLHLAAVFACNFSNYCFDMAKQVVDAEQVDFGLLYPLILETAKKATENDPKQMQTGPAMRGDQNILVMHQSLLAQANRDDLKQVYQLLSDGIVKRHHSS 3DFU Chain:B ((7-219)) LRVGIFDDGSSTVNMAEKLDSVGHYVT-VLHA-------------------PEDIRDFELVVIDA--HGVEGYVEKLSAFARRGQMFLHTSLTHGITVMDPLETSGGIVMSAH---PIG--------QDRWVASALDELGETIVGLLVGELGGSIVEIADDKRAQLAAALTYA-GFLSTLQRDASYFLDEFLGDPDV-------TS-----DIVMDSAQQFQALPSLDEVIAQYDSINN--PG-RQRLFRDLARRQAEISRA-

General information: TITO was launched using: RESULT: Template: 3DFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 826 -78877 -95.49 -377.40 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -95.49 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.309

(partial model without unconserved sides chains): PDB file : Tito_3DFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DFU-query.scw PDB file : Tito_Scwrl_3DFU.pdb: