Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTRTVQTLKNSTDLVQRFALPEIWEDFELRRLSNKDRYKHYILIFKNVVSQKKDWEDVKVVAEIQERNHNLRFNIKISKQYPELAAYEKLLEAKINAIINQSSLVIS 2GIB Chain:A ((3-99)) -------NVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPP----

General information: TITO was launched using: RESULT: Template: 2GIB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 255 -9391 -36.83 -96.81 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -36.83 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.103

(partial model without unconserved sides chains): PDB file : Tito_2GIB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GIB-query.scw PDB file : Tito_Scwrl_2GIB.pdb: