Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVIVLGSGVIGVASAYYLARQGAEVTVLDRQSGPAEETSFGNAGQISPGYSTPWAAPGIPFKAVKWMFQHHAPLAINLDGSMWQLQWMAQMLKNCNPQSYAVNKERMMRVAEYSRDCLRELRKDTGIHYENRAKGTLQLFRKEAQMEAVQRDISVLEECGVSYELLNGNELGRVEPALANAQDKLVGGLHLPNDETGDCYLFTNALAQIAKELGVNFQFNQNVEKLIVEGDQIKGVQVNGKVLTADRYVLAFGSYSRDFLKPLDLQLPVYP---VKGY------SLTIPIVAPAFAPQSTVLDETY-----------KIAITRFDQRIRVGGMAELSGFNLGLNEDRHATLQMVTQDLFPGGDMEQASFWTGLRPMTPDSTPIIG-ATRFKNLFLNTGHGTLGWTMACGSGKLISDIVLNHKTDISTDGLSIQRYSHAHAA 1L9D Chain:A ((6-377)) --VIVVGAGSMGMAAGYQLAKQGVKTLLVDAFDPPHTNGS------------------------------HHGDTRIIR-------------------HAYGEGRE-YVPLALRSQELWYELEKET--HHKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVPEN--YNAIFEPNSGVLFSENCIRAYRELAEARGAKVLTHTRVEDFDISPDSVKIETANGS-YTADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDIDFPGFMVE--VPNGIYYGFPSFGGCGLKLGYNTFGQKIDPDTINR----EFGVYPEDESNLRAFLEEYMPGANGELKRGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDISIFSINR-------

General information: TITO was launched using: RESULT: Template: 1L9D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2181 -115161 -52.80 -328.09 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -52.80 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1L9D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L9D-query.scw PDB file : Tito_Scwrl_1L9D.pdb: