Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQDHIEQHRRYIVISYAFMFLALFTVIFAAFAYLVARKVAVVDDAEVWIHAHALW----IMRNGILFLLMSVFAVAWFIPLFFFAWDSNLWVTASTVAGVVFSAIAWLFLLNAWLKGLSKYLKNKAVF 2N3Z Chain:A ((1-99)) MG-------RLVVVVTS----EQLKEEVRKKFPQVEVRLVTTEEDAKQVIKEIQKKGVQKVVLVGVSEKLLQKIKQEANVQVYRVTSNDELEQVVKDVKG----------------SGLEHHHHHH---

General information: TITO was launched using: RESULT: Template: 2N3Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 410 -53616 -130.77 -564.38 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -130.77 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_2N3Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N3Z-query.scw PDB file : Tito_Scwrl_2N3Z.pdb: