Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSLSLLHPYPFEKLNQLFKDTTPANLPLIPLSIGEPKHPAPEFVKQAIIDNFNHLST-YPNSKGLPELRQSIADWLTKRFKLNSISAENHILPVSGTREGIFSFVQTLINREDAPYVVMPNPFYQIYEGAALLAGAKPYFINCTEENGYLGDFDAVPAEVWEKTALLFVCTPGNPTGTVLSKEQFKKLIALSDQYNFVIASDECYSELWFDQ---APTGLLEVCAELGRGDYKNCIVFHSLSKRSNLPGMRSGFIAGDAALLKPYLQYRTYHGAAMPVQHQLASIAAWNDE----SHVEENRKQYRAKFDLFQSELGHL-LPLQKPDAGFYYWLK---VDNDETFAKMLMEKAHIKVLPGRYLSRDTEQGNPGANHVRMALVADLAQCEEVVKRLKAIL 1GC3 Chain:A ((33-382)) -----------------------------LVALTAGEPDFDTPEHVKEAARRALAQGKTKYAPPAGIPELREALAEKFRRENGL-SVTPEETIVTVGGS-QALFNLFQAILDPGDE--VIVLSPYWVSYPEMVRFAGGVVVEVETLPEEGFVPDPERVRRAITPRTKALVVNSPNNPTGAVYPKEVLEALARLAVEHDFYLVSDEIYEHLLYEGEHFSP----------GRVAPEHTLTVNGAAKAFAMTGWRIGYACGPKEVIKAMASVSRQSTTSPDTIAQWATLEALTNQEASRAFVEMAREAYRRRRDLLLEGLTALGLKAVRPSGAFYVLMDTSPIAPDEVRAAERLLEAGVAVVPGTDFA--------AFGHVRLSYATSEENLRKALERFARVL

General information: TITO was launched using: RESULT: Template: 1GC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1958 -4485 -2.29 -13.27 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -2.29 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_1GC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GC3-query.scw PDB file : Tito_Scwrl_1GC3.pdb: