Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTFTLFFGVPSGCSFGSIVALEWSGLPYQLCRIEMPAQCNTEEYRQINPVGETPALLYDNGKVLTESMAILNHIMAKSLNAAFHYEQGTEQFNLFNQKLAFLNTTLFNAFSPHWYSLEHSSSEGNKQALREYGAVQVKKAFTALENSLTNQTWLMGDQLSFVDGYYAGIARWLKYHNVIDIDQFPNCKRLYEQLQNEAAVKFAHAIEQE 3EIN Chain:A ((43-185)) -------------------------------------------EFLKINPQHTIPTLV-DNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFK-----KIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAK-FEISKYANVNRWYE-----------------

General information: TITO was launched using: RESULT: Template: 3EIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 514 -81182 -157.94 -567.70 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -157.94 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_3EIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EIN-query.scw PDB file : Tito_Scwrl_3EIN.pdb: