Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQYQPPHINRKLNVQAWWLTALLISINVGLFIWQIISGVDISDPAIKDALHWGADFTPLTFSGQPERLFTSMFFHFGIIHLLLNMWALYIFGNVAEALFGRLYFIGLYLLAGLFGSLLSSYINIQNGHELLQHFDQSLLPHVSAGASGAVMGLGAALTVLSLFPPLPHQAYILDKKALLMIMAINLI---FGFVATGINNAAHVGGMIMGALLALIWYLSYRTPFKSLLKFLGLVGGLFMTVGFYVYCNQLNSPLLPLWHEVLIQNPEILP 2NRF Chain:A ((127-267)) --------------------------------------------------------FDP-TLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGS----GKLIVITLISALLSGYVQ--------QKFSGPWF----GGLSGVVYALMGYVWLRGERDP---QSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFV-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2NRF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 532 -101637 -191.05 -736.50 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -191.05 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_2NRF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NRF-query.scw PDB file : Tito_Scwrl_2NRF.pdb: