Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLLKVCTLLPCSLVIAACSVNSSSTPPTLSSSTPQVQKSSIEISCQDLQNPAYRQAVLNAINQIRQDSRQCGQ----QYF---SAAKPLSWNNNLYQGANAHSKDMANNNFLGHVGSTGLDLRARLKKYHMLSKANGENVASG---------QKTLNEVLAKWI----------------ASPLHCSNIMNPRYTEYAIACASDQSAKQRSYWTQQFAGF 3NT8 Chain:B ((33-178)) --------------------------------------------------TDSDRQAFLDFHNNARRRVAKGLEDSNSGKLNPAKNMYKLSWDCAMEQQLQDAIQSCPSG----FAGIQ----------------GVAQNTMSWSSSGGYPDPSVKIEPTLSGWWSGAKKNGVGPDNKYTGGGLFAFSNMVYSETTKLGCAYKVCG---TKLAVSCIYN--

General information: TITO was launched using: RESULT: Template: 3NT8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 259 4388 16.94 38.49 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 16.94 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_3NT8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NT8-query.scw PDB file : Tito_Scwrl_3NT8.pdb: