Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHLHVHPENPQQRLIEQAVERIRAGDVVVYPTDAAYAIGCQIGNKNAMERIAQIRGLGPKHQYAIMCCDLSDIATYAK---VDNATYRLLKANTPAITTFILPATSEVPRRLMHPKKKTIGLRIPSNPICQALLKELGGPLLTSTLILPGQEDPLDDPYDIENQLAKRIDVFIDGGFGTLTTTSIVDLSGEHPEIIRHGVGDVSAFE 1HRU Chain:B ((4-183)) ---------NLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVMRLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPATTPRWLT-GRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPC-RTVDEVRAQFGAAFPVVPGETGGRLNPSEIRDALT-----------------

General information: TITO was launched using: RESULT: Template: 1HRU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 898 -51626 -57.49 -291.67 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -57.49 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_1HRU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HRU-query.scw PDB file : Tito_Scwrl_1HRU.pdb: