Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDHIAQPISYQHIELSFPVHLDIKRLDLVHP-QISGNKFFKLKYNLLAAKEQGLSSILTFGGAYSNHIAATAYAAHLFGLKSIGIIRGEELAGKPLNPTLAKAQSLG-MQLHFVSRHEYRL---RDEANYLKQLQQQFPQTYIIPEGGTNE----LAVQGCQEILSQYDLE---QYD-VICCAVGTGGTISGLIERSAAH---QKVLGFSA-LKGDFLQQEIKQWTK-------------KQNWSLTDVYCWGGYAKTSPELFTFIENFEEQYTVPLEPIYTGKMMFGLFDLIKNNYFPANTRILAIHSGGLQADIRNRSHA 1TZK Chain:A ((19-327)) ------QPLARLSKHLGGKVHLYAKREDCNSGLAFGGNKTRKLEYLIPEALAQGCDTLVSIGGIQSNQTRQVAAVAAHLGMKCVLVQE------NWVNYSDAVYDRVGNIQMSRILGADVRLVPDRSWEDALESVRAAGGKPYAIPAGCSDHPLGGLGFVGFAEEVRAQEAELGFKFDYVVVCSV-TGSTQAGMVVGFAADGRADRVIGVDASAKPAQTREQITRIARQTAEKVGLERDIMRADVVLDERFAGPEYGLPNEGTLEAIRLCARTEGMLTDPVYEGKSMHGMIEMVRNGEFPEGSRVLYAHLGGVPA--------

General information: TITO was launched using: RESULT: Template: 1TZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1478 11553 7.82 42.47 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 7.82 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_1TZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TZK-query.scw PDB file : Tito_Scwrl_1TZK.pdb: