Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENNLILPALILVPFIAGFTCWLVDKLDKTLPRWIALIGMLITLGLGLALWQSGTYSYELGSKVPTWTAEFYLPWIQSLGIGIHLAVDGLSLLMVLLTALLGVLAVGCSWGEIQKNVGFFHLNLLWSLGGVIGVFLAIDLFLFFFFWEMMLVPIYFLIALWGHKGSNGRSRVYAATKFFLYTQIAGLVMLIGILGLVVYGYMMTGMIGFDYNYLLAVANRLPAGFAYGFMICFFIGFAVKLPVFPLHGWLPDAHAQAPTAGSVDLAGILIKTAAYGLLRFVIPFFPAASAQFADIAIIFGLIGIFYGAWCAYQQTDMKRLLAYTSISHMGFVLLAIYAGNILTFQGLMIMMLAHGLSSAALFIMSGQIYERLHTRDLRLMGGLRGQLQYLPFFLMFFVAALIGVPGLGNFIGEFLILMGSFNKYPVFTIIASISLVFAGLYGLILIHRALFGEPNSEQKQHYTSPLKDLSAREVSILMICAIGLVWLGIYPQTFLDVSHSSMQWLVNSYVPVQEFVETVQQTATQLDHVEIQ 4HE8 Chain:G ((2-463)) ------VVLAVLLPVVFGALLLLGLP------RALGVLGAGLSFLLNLYLFLTHPG---------GVAHAFQAPLLPGAGVYWAFGLDGLSALFFLTIALTVFLGALVAR-----VEGRFLGLALLMEGLLLGLFAARDLLVFYVFFEAALIPALLMLYLYGG-----EGRTRALYTFVLFTLVGSLPMLAAVLGARLLSGS----PTFLLEDLLAHPL--QEEAAFWVFLGFALAFAIKTPLFPLHAWLPPFHQENHPSGLADALGTLYKVGVFAFFRFAIPLAPEGFAQAQGLLLFLAALSALYGAWVAFAAKDFKTLLAYAGLSHMGVAALGVFSGTPEGAMGGLYLLAASGVYTGGLFLLAGRLYERTGTLEIGRYRGLAQSAPGLAALALILFLAMVGLPGLSGFPGEFLTLLGAYKASPWLAALAFLSVIASAAYALTAFQKTFWEEGGSGV--------KDLAGAEWGFALLSVLALLLMGVFPGYFARGLHPLAEAFAK-------------------------

General information: TITO was launched using: RESULT: Template: 4HE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 2850 -538976 -189.11 -1166.61 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain G : 0.77 3D Compatibility (PKB) : -189.11 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_4HE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HE8-query.scw PDB file : Tito_Scwrl_4HE8.pdb: