Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMEEHLLTQKLQQRLRFSTHIQEAQAAVLIAITNENNPKVLLTRRSIHMNNHAGEVSFPGGK----RDPSDTSNIVVALREAQEETALNPFDVQLLGDLPMQRARSGLSVKPIVGLIPPE--VTLIPQPT-EIDRIFFVPLQQLIEMRPTPYEVRYAHQSLYFPSLQIDNEIIWGLTAQMLIALFKYGLGYQKEWPFLLNAPTFGMPKFSHKK 1SJY Chain:A ((7-145)) --------------------THVPVELRAAGVVLLNERGDILLVQEKGI---EKAGLWHIPSGAVEDGENPQDA-----AVREACEETGLRVRPVKFLGAY-LGRFPDGVLILRHVWLAEPEPGQTLAPAFTDEIAEASFVSREDFAQLYAAG-QIRMYQTKLFY--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1SJY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 560 -6618 -11.82 -51.70 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -11.82 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_1SJY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SJY-query.scw PDB file : Tito_Scwrl_1SJY.pdb: