Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDEEDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYT---SDDGFSVVNYQAINDRLGTWEDIEEM-----RSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDTDTSSVTRPRTSPLLTTFIDKNG-------KEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLN------PVRGI--LPEKTILE--LVEQLKKEGAR-VNYKKNPDGTTSPYEINATYLDALSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRND---YKGVEKTGENRKINRQKFLLADIEKELQTDPLRTA--VYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEEKVLVIHNFS---NQSAGYLLPKGDY--LDLFSGQIIHCSQERKLIFQPYEYMWLKIIK 4FLO Chain:A ((130-627)) ---------------------------------------------------------------------------------LHLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRT----LREIFPDQHPGGFSQLEDGRWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVPCIWKQMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSEAIV-HPDQVVQY-IGQDECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFQYNP--STGDCRVSGTAAALVGLAQDDPH-AVDRIKLLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQ--RNDPSTAAGQIYQDLRHMIAVRQSNPRFDGGRLVTFNTNNKHIIGYIR---NNALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVSLNQD--LTLQPYQVMWLEI--

General information: TITO was launched using: RESULT: Template: 4FLO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2476 16182 6.54 35.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 6.54 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_4FLO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FLO-query.scw PDB file : Tito_Scwrl_4FLO.pdb: