Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EWCTVTVFYQGRTSGYPLPTAKKVGIVQSFPGEGKGYADYQYILDENCDWKEHGRPWLTGMRLETVPFVAKQKYE---------------------------------------------------------------------------------- 3WL8 Chain:A ((23-358)) VKWECPAGYEVKEGLNVDFPHKGMKRAFIVYPAKNVSGPAPVWVPMTGSVESTNDNLTVARSGANSILADHGYTVIAPVRACANQDPNIRGERCNGPGSNGWNWNPWFEGRAADPSGEHWKNDEGPDSSFFVAMVQCVGTKYKLDARRLFLGGIASGGTMTNRALLFRSNFWAGGLPISGEWYVTSDDGTPLSFDDARAAVAAAPTKIHQGRVGPYPLPAKVGPLIVMTVWGGEK-------------DLWNCTRP--DGSRFLCAD------YRPSTQAGSNFFSAQPDVVHVACSSTHGHMWPQLNTQEFNRWALDTLASHPKGSDPRSFKLTQPPEGYTCHVGPFTGLYASAWS

General information: TITO was launched using: RESULT: Template: 3WL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 82 -2759 -33.64 -51.08 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -33.64 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.034

(partial model without unconserved sides chains): PDB file : Tito_3WL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WL8-query.scw PDB file : Tito_Scwrl_3WL8.pdb: