Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNK-------------TVFKAS--SFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKIL----------APAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWK--------------------------------------HP--FFLDDRRTPAKMCLNRTS---QENISFETMYDVLSTK----PVL------NKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 5GS7 Chain:B ((1-289)) ----------------------------------------------------------------------------------------------------------------------------------------------ATAITYVS--KDHYFGRNFDYEISY---------------NE--VVT-ITPRNYKFSFREVGNLDHHFAIIGIAAGIADYPLYYDAINEKGLGMAGLNFSGY-ADY---KKI-EEGKENVSPFEFIPWVLGQCSTVDEAKKLLKNLNLVNINFSDELPLSPLHWLLA-DKE-QSIVVESTKEGL-RVFD------NPVGVLTNNPTFDYQLFNLNNYRVLSTRTPKNNFSDQIELDIYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSRSSEYESISQFFHILSSVEQQKGLCDVGDEKYEYTIYSSCCNLEKGIYYYRTYD--------

General information: TITO was launched using: RESULT: Template: 5GS7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 985 14674 14.90 69.55 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : 14.90 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_5GS7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GS7-query.scw PDB file : Tito_Scwrl_5GS7.pdb: