Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYFGKIGRKDAERQLLSPGNPQGAFLIRESETTKGAYSLSIRDWDQTRGDHVKHYKIRKLDMGGYYITTRVQFNSVQELVQHY 1SPR Chain:A ((5-87)) WYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYY

General information: TITO was launched using: RESULT: Template: 1SPR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 309 -40623 -131.46 -489.43 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -131.46 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.720

(partial model without unconserved sides chains): PDB file : Tito_1SPR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SPR-query.scw PDB file : Tito_Scwrl_1SPR.pdb: