Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KSLSTGELEEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHY 4WAF Chain:B ((47-120)) --------EEVNEKLRDTADGTFLVRDAST----DYTLTLRKGGNNKLIKIF-------FSDPLTFSSVVELINHY

General information: TITO was launched using: RESULT: Template: 4WAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 156 -26758 -171.53 -469.44 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -171.53 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.832

(partial model without unconserved sides chains): PDB file : Tito_4WAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WAF-query.scw PDB file : Tito_Scwrl_4WAF.pdb: